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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + <t>SRT2104</t> groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.
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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + <t>SRT2104</t> groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.
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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + <t>SRT2104</t> groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.
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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + <t>SRT2104</t> groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.
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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + <t>SRT2104</t> groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.
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SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + SRT2104 groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + SRT2104 groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Article Snippet: Mice in the L-NAME + SRT2104 group additionally received intraperitoneal SRT2104 (25 mg/kg/day; Cat. No. HY-15262; MedChemExpress, Monmouth Junction, NJ, United States), as previously described ( ).

Techniques: Control, Staining, Comparison